Effects of Sinomenine on Proliferation of Human Fibroblast-like Synoviocyte and Expression of MMP-3 mRNA from Patients with Rheumatoid Arthritis
- VernacularTitle:青藤碱对类风湿关节炎患者成纤维样滑膜细胞增殖及基质金属蛋白酶-3表达的影响
- Author:
Juan ZHANG
;
Juan LI
;
Yi ZHAO
- Publication Type:Journal Article
- Keywords:
rheumatoid arthritis;
sinomenine;
fibroblast-like synoviocytes;
MMP-3
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of sinomenine on the proliferation of fibroblast-like synoviocytes (FLS) and expression level of MMP-3 secreted by FLS in rheumatoid arthritis in vitro. Methods FLS were obtained by digesting synovial tissues with collagens and were divided into four groups:sinomenine high, middle, low concentration and control group. The proliferation of FLS was assessed by methyl- thiazolyl-tetrazolium (MTT) assay and the mRNA expression of MMP-3 was measured by semi-quantitative RT-PCR respectively after treated by sinomenine. Results The rate of FLS proliferation was significantly reduced in groups treated by sinomenine. Compared with control group, the mRNA expression of MMP-3 markly decreased in sinomenine groups (P
