Determination of Icariin in Jiawei Wuzi Yanzong Pill by HPLC
- VernacularTitle:高效液相色谱法测定加味五子衍宗丸中淫羊藿苷含量
- Author:
Minfang LI
;
Hui LI
;
Xuemei WANG
- Publication Type:Journal Article
- Keywords:
Jiawei Wuzi Yanzong Pill;
icariin;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of content determination of icariin in Jiawei Wuzi Yanzong Pill. Method The HPLC method was used to determine the content of icariin from Jiawei Wuzi Yanzong Pill. The analysis was carried out on Lichrospher C18 column (250 mm?4.6 mm, 5 ?m) with acetonitrile-water (30∶70) as mobile phase, flow-rate of 1.0 mL/min and wave-length at 270 nm at 30 ℃. Results The liner range of icariin was 0.28～2.60 ?g with a correlation(r) of 0.999 9. The recovery was 100.86% with RSD of 0.71%. Conclusion The method had good reproducibility and sensitivity to determine the content of icariin from Jiawei Wuzi Yanzong Pill.
