Effect of Quercetin on Proliferation of Human Breast Carcinoma Cells
- VernacularTitle:槲皮素对人类乳腺癌细胞增殖的影响
- Author:
Lixia SHEN
;
Huiyu XU
;
Piwen ZHAO
- Publication Type:Journal Article
- Keywords:
phytoestrogen;
quercetin;
estrogen receptor;
cell proliferation;
MCF-7;
MDA-MB231
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of quercetin on the proliferation of mammary cancer cell in vitro. Methods Effects of quercetin on the cell proliferation was tested in ER-positive MCF-7 cell and ER-negative MDA-MB231 cell by MTT measurement. And to evaluate the estrogen-like effect of quercetin and its relation with the estrogen-receptor by pure estrogen receptor antagonist ICI 182, 780 as a tool. Cell cycle was examined by flow cytometry. Result Quercetin (10～50 ?mol/L) stimulated proliferation of ER-positive MCF-7 cell compared with solvent control, whereas ER-negative MDA-MB231 cell proliferation effect was inhibited, and the cell cycle was impulsed from G1 to S, DNA synthesizing was inhanced, PI was also increased. The above function on boosting MCF-7 cell proliferation can be inhibited by adding estrogen receptor antagonism ICI182, 780. Conclusion Quercetin has the estrogen-like activities through the estrogen response pathway.
