Ultrafiltration and nanofiltration membrane separation technology in extracting and purifying oligosaccharides of Rehmannia glutinosa
- VernacularTitle:超滤和纳滤分离技术提取纯化地黄低聚糖的研究
- Author:
Yan DONG
;
Ruichang GAO
;
Qin PAN
;
Xiaonan YAN
- Publication Type:Journal Article
- Keywords:
the oligosaccharides of Rehmannia glutinosa Libosch;
ultrafiltration(UF);
nanofiltration(NF);
separation;
purification
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective Oligosaccharides of Rehmannia glutinosa were separated and purified by different molecular weight cut-off ultrafiltration(UF) membranes,and then ultrafiltrate was concentrated and purified by nanofiltration(NF) membrane.Methods Different molecular weight cut-off UF unit first and then NF unit were used in process.Results Oligosaccharides were separated by twice UF,the optimum separation conditions:the concentration of feed solution was 13—132 mg/mL,the operation pressure was 0.25—0.275 MPa,and the temperature was 20—40 ℃.Then ultrafiltrate was concentrated and purified by NF membrane,the optimum separation conditions:the operation pressure was 0.59—0.79 MPa,the temperature was 20—40 ℃,and the concentration multiplegot to three.Under this condition,the total extraction rate of oligosaccharide products was 46.63% and the purity was above 93.3%,the molecular weights of oligosaccharide products determined by gel filtration were less than 6 000.Conclusion The technology is not only simple and feasible but also easy to separate and purify the oligosaccharides of R.glutinosa effectively.
