Effects of matrine on proliferation and metastasis abilities of human ovarian carcinoma HO-8910PM cells line
- VernacularTitle:苦参碱对人卵巢癌细胞株HO-8910PM增殖及侵袭运动能力的影响
- Author:
Zhihua SHEN
;
Wei JIE
;
Jin CHEN
;
Peichun HUANG
- Publication Type:Journal Article
- Keywords:
matrine;
ovarian carcinoma;
proliferation;
invasion;
cytoskeleton;
Ezrin
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of matrine on proliferation and metastasis associated the abilities of human highly metastatic ovarian cancer HO-8910PM cells and its possible mechanism.MethodsThe trypan blue staining and MTT assay were used to examine the effect of matrine on proliferation of HO-8910PM cells;Transwell chamber assay was performed to determine the effect of matrine on the invasive and migratory abilities of the cells;Coomassie brilliant blue staining assay was used to detect the changes in cytoskeleton treated by matrine at different concentrations and immunocytochemical methods were used to observe the expression of Ezrin protein in HO-8910PM cell line befores and after matrine treatment.Results There was an obvious dose-dependent and time-consuming effect on the inhibition of HO-8910PM cells proliferation by matrine;1.5 g/L matrine inhibited cell proliferation significantly,for 6 h after the treatment in vitro,the invasive and migratory abilities of HO-8910PM cells were also obviously down-regulated with the inhibitory rates(41.52?10.76)% and(39.73?10.67)%,respectively;Matrine distinctly changed the cytoskeleton and up-regulated the expression of Ezrin protein in HO-8910PM cell line treated with 1.0 g and 1.5 g/L matrine for 24 h.Conclusion Matrine inhibits the proli-feration,migration,and invasion abilities of HO-8910PM cell line in vitro,which may associate with the changes of cytoskeleton and Ezrin protein expression.
