Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell
- VernacularTitle:脂质体转染survivin siRNA对A549细胞增殖及凋亡的影响
- Author:
Yongquan LIU
- Publication Type:Journal Article
- Keywords:
Survivin,siRNA;
Cell apoptosis;
Lung carcinoma;
RNAi
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of liposomal transfection of survivin siRNA on the proliferation and apoptosis of lung adenocarcinoma cell lines A549.Method:Survivin siRNA was synthetized and transfected into A549 cell by liposome.Cell morphological change was observed with inverted fluorescent microscope,cell proliferation was examined by MTT assay at 24h 48h and 72h after transfection.cell proliferation and apoptotic index detected by flow cytometry and survivin mRNA expression determined by RT-PCR.Results:For the A549 cell transfected with siRNA cell proliferation was ihibited significantly,cell apoptosis appeared obvious and the expression of survivin mRNA significantly decreased.Conclusion:Survivin siRNA is able to inhibit the proliferation of lung adenocarcinoma cell lines A549 and induce the cell apoptosis.Survivin might become a new target for lung carcinoma gene therapy.
