Establishment and analysis of 2-DE patters of cytoplasmic proteomics of immortalized cervical cell and cervical cancer cell
- VernacularTitle:宫颈永生化细胞和癌细胞的胞质蛋白双向电泳图谱的建立及初步分析
- Author:
Daping SONG
- Publication Type:Journal Article
- Keywords:
Cervix;
Human immortalized endocervical cell;
Cancer cell;
Proteomics;
Two-dimensional gel electrophoresis(2-DE)
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from immortalized human endocervical cell(H8) and cervical cancer cell(CaSKi),and to identify the differential expressions of cytoplasmic proteins.Methods:H8 cells and CaSKi cells were incubated,the cytoplasmic proteins of H8 cells and CaSKi cells were extracted and seperated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).The differential expression proteins were analyzed by using ImageMaster 2D analysis software.Results:We obtained well-resolved,reproducible 2-DE patterns,and 8 notable differential protein spots were defined in 2-DE gels.Conclusion:Cytoplasmic proteins of cervical precancerous lesion and cervical cancer are separated by means of immobilized pH gradient-based two-dimensional gel electrophoresis(2-DE).The expression maps of proteomics in cytoplasmic proteins of H8 and CaSKi are established.There are significant differences in proteome between cytoplasmic proteins of H8 cells and CaSKi cells.These data may be valuable for differential proteomics research of cervical precancerous lesion and cervical cancer.
