Effect of Radix Rehmanniae on expression of collagen Ⅰ and Ⅲ of pulmonary interstitial fibroblast in rat
- VernacularTitle:生地对大鼠肺间质成纤维细胞Ⅰ、Ⅲ型胶原表达的作用
- Author:
Li LIU
;
Lan TANG
;
Desheng XU
;
Qi CHEN
- Publication Type:Journal Article
- Keywords:
Radix Rehmanniae;
oligosaccharid;
collagen Ⅰ;
collagen Ⅲ
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of Rehmannia glutinosa Libosch on collagen Ⅰ and Ⅲ of in pulmonary interstitial fibroblast in rat. METHODS: The components of Radix Rehmanniae were extracted and isolated to several parts.Primary pulmonary fibroblasts were separated from lung interstitial tissue of rat.After being reproduced for several generations,fibroblasts were cultured in the extracts of Radix Rehmanniae and Shengdi Solution for injection in several concentrations respectively for 72 hours,and then contents of Col Ⅰ and Col Ⅲ of these fibroblast were determined by RT-PCR. RESULTS: The part containing mainly oligosaccharide from preparation that decocted with water and deposited with alcohol could inhibit Col Ⅰ with a few exceptions concentration while it could inhibit Col Ⅲ at high concentration.The part of deposit inhibited Col Ⅰ obviously in the concentration of 2?10~(-2) g/mL and could inhibit Col Ⅲ in all the concentrations.Shengdi Solution for injection could inhibit Col Ⅲ in all the concentrations. CONCLUSION: Some constitutents of Radix Rehmanniae can inhibit the expression of Col Ⅰ and Col Ⅲ of pulmonary fibroblast which is one of mechanisms that Radix Rehmanniae takes effect on fibrosis disease in pulmonary interstitial tissue.
