Determination of geniposide and scutellarin in Yinshanlian Granules by HPLC
- VernacularTitle:HPLC同时测定茵山莲颗粒中野黄芩苷和栀子苷含量
- Author:
Xingchao LIU
;
Jinyu SONG
;
Haiyan XU
;
Hongmin ZHU
;
Jinyu MEN
;
Huaiqing ZHAO
- Publication Type:Journal Article
- Keywords:
Yinshanlian Granules;
scutellarin;
geniposide;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a HPLC method for determining scutellarin and geniposide in Yinshanlian Granules(Herba Artemisiae scopariae,Herba Scutellariae barbatae, Fructus Gardemiae,etc). METHODS: The analysis was performed on Diamonsil C_(18)(200 mm?4.6 mm,5 ?m) with mixture of acetonitrile(A) and 0.1% phosphoric acid solution as mobile phase in gradient mode.The concentration of solvent A were 5%,33%,5% and 5% at 0,30,31 and 35 min,respectively.The detection wavelength was set at 240 nm and the column temperature was at 30 ℃.(RESULTS:)The linear calibration curves were obtained in the concentration range of 0.032-0.288 mg/mL for scutellarin and 0.009 6-0.086 4 mg/mL for geniposide.The average recoveries of scutellarin and geniposide were 100.6%(RSD=0.80%,n=9),102.6%(RSD=1.1%,n=9),respectively.CONCLUSION: The method is quick,simple,and reproducible,which can be used to control the quality of Yinshanlian Granules.
