Determination of notoginsenoside R_1,ginsenoside Rg_1,Rb_1 in Jingkang Capsules by HPLC
- VernacularTitle:HPLC测定颈康胶囊中人参皂苷Rg_1、Rb_1与三七皂苷R_1的含量
- Author:
Ning ZHANG
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Jingkang Capsules;
notoginsenoside R_1;
ginsenoside Rg_1,Rb_1;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a method for determining notoginsenoside R_1,ginsenoside Rg_1 and ginsenoside Rb_1 in Jingkang Capsules(Radix Rehmanniae praeparata,Radix Polygoni multiflori,Radix et Rhizoma Notoginseng,etc.). METHODS: A Diamonsil C_(18) column(250 mm?4.6 mm,5 ?m) with acetonitrile-water as the mobile phase was used for gradient elution,the flow rate was 1.0 mL/min and the UV detection wavelength was at 203 mn. RESULTS: There was a good linear relationship at a range of 0.327-2.180 ?g for notoginsenoside R_1(r=(0.999 4))(the average recovery was 98.23% with RSD of 1.53%(n=6)),0.909-6.060 ?g for ginsenoside Rg_1(r=1.0)(the average recovery was 99.04% with RSD of 1.00%(n=6)),0.672-4.480 ?g for ginsenoside Rb_1(r=0.999 8)(the average recovery was 98.17% with RSD of 1.31%(n=6)).CONCLUSION: The method is simple,quick and accurate.It can be used for the quality control of Jingkang Capsules.
