RP-HPLC Determination of Matrine in Fuji Lotion
- VernacularTitle:反相高效液相色谱法测定肤疾洗剂中苦参碱的含量
- Author:
Ning ZHANG
;
Xiaofan MA
- Publication Type:Journal Article
- Keywords:
Fuji Lotion;
matrine;
RP-HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of matrine in Fuji Lotion. Methods An Inertsil NH2 column (250 mm?4.6 mm, 5 ?m) was used with acetonitrile-ethanol absolute-3% phosphoric acid (83∶8.5∶8.5) as mobile phase, the flow rate was 1.0 mL/min, and the UV detection wavelength was set at 220 nm. Results The calibration curve of matrine was linear in range of 0.039～1.560 ?g, r=0.999 7. The average recovery was 97.8% with RSD of 1.1% (n=6). Conclusion The method is simple, quick and accurate. It can be used for the quality control of Fuji Lotion.
