Determination of Puerarin Content in Jingning Capsule by HPLC
- VernacularTitle:高效液相色谱法测定颈宁胶囊中葛根素的含量
- Author:
Ruju XIONG
;
Xinfei MA
;
Tulin LU
- Publication Type:Journal Article
- Keywords:
Jingning capsule;
HPLC;
puerarin
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of puerarin content in Jingning capsule. Methods The column was Kromasil (250 mm?4. 6 mm, 5 ?m). The mobile phase consisted of methanol- 0.2% water H3PO4 (20/80), with flow rate of 1 mL/min and the UV detective wavelength set at 250 nm. Results Puerarin showed a good linear at the range of 6.268～125.360 ?g/mL (r=0.999 8). The average recovery was 101.42%, with RSD of 1.22%. Conclusion This method is accurate, simple, repeatable and suitable for the determination of puerarin content in Jingning capsule.
