Effects of Serum Containing Qiangjijianli Oral Liquid on In-Vitro Proliferation of Rat Mesenchymal Stem Cells
- VernacularTitle:强肌健力口服液含药血清对大鼠骨髓间充质干细胞体外增殖的影响
- Author:
Kaijia CHEN
;
Xiaobin LIU
;
Shijun QIU
- Publication Type:Journal Article
- Keywords:
invigorating the spleen;
Qiangjijianli oral liquid;
mesenchymal stem cells;
serum containing drug
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of serum containing Qiangjijianli oral liquid in-vitro proliferation of rat mesenchymal stem cells (MSCs). Methods Rat mesenchymal stem cells dissociated from the bone marrow by density gradient method were cultured. MSCs were identified by marking of bromodeoxyuridine (Brdu) and staining of CD44, CD45. Experimental group was cultivated with serum containing Qiangjijianli oral liquid and control group with blank serum. Optical absorption value of MSCs was stained by methyl thiazolyl tetrazolium (MTT). Results Serum containing Qiangjijianli oral liquid at different concentrations could promote the proliferation of MSCs, the difference being significant in comparison with the control group (P
