THE REGULATION OF EXOGENOUS POLYAMINES ON ODC PROTEIN LEVEL IN THE EARLY REGENERATING LIVER OF RATS
- VernacularTitle:外源多胺对大鼠早期再生肝鸟氨酸脱羧酶蛋白水平的调节
- Author:
Juntao HE
;
Jianmin MA
;
Qianji NING
- Publication Type:Journal Article
- Keywords:
Polyamine;
Ornithine decarboxylase;
Regenerating liver;
Western blotting;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of polyamines(putrescine,spermidine and spermine) in regulating ornithine decarboxylase(ODC) protein levels in the early regenerating liver of rats. Methods Exogenous polyamines(dissolved in 0.9% NaCl) were administered subcutaneously to adult male SD rats(180～220g).The ODC protein levels in the regenerating liver induced by partial hepatectomy(PH) were measured by Western blotting analysis. Results The treatment with high doses of putrescine( 20 mg/kg body weight),spermidine(0.15 mg/kg body weight) or spermine(6 mg/kg body weight) reduced the ODC protein level compared with the control group within 12 hours after PH.However,the treatment with low doses of putrescine(0.02 mg/kg),spermidine(0.03 mg/kg) or spermine(0.06 mg/kg) resulted in a rapid increase in the protein level in the 4th hour after PH,which was 15.1%,29.5% or 30.3% higher than that of control rats respectively.Conclusion Polyamines with certain doses play a role in the feedback regulation of ODC protein levels in the early regenerating liver,where spermidine and spermine play a greater role than putrescine dose.
