HPTLC Chromatographic Fingerprint Analysis of Bupleurum spp.(Chaihu)
- VernacularTitle:柴胡属药材皂苷高效薄层色谱指纹图谱的研究
- Author:
Heping LIU
;
Peishan XIE
;
Runtao TIAN
- Publication Type:Journal Article
- Keywords:
Bupleurum chinense DC.;
Saikosaponin;
Thin-Layer Chromatography;
Chromatographic fingerprints;
Species identification;
Quality evaluation
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The High-performance thin-layer(HPTLC) chromatographic fingerprint of Saikosaponin from Chaihu(roots of Bupleurum chinense DC.) was established and fingerprints similarity of different species of Bupleurum spp.were evaluated.Methods High-performance thin-layer chromatography(HPTLC)were carried out.The chromatographic conditions were as follows:pre-coated HPTLC silica-gel plate as stationary phase,dichloromethane-methanol-ethyl acetate-water(30:40:15:3) as mobile phase(solvent system) and 2 %p-DMBA/10 %Sulfuric acid alcoholic solution as derivatization reagent.The common pattern of HPTLC fingerprints were obtained through’Chromafinger’solution software,and authentication and quality assessment were analyzed by similarity and Principle Component Analysis.Results The common pattern of the roots of Bupleurum chinense DC.consists of 19 characteristic peaks,and higher similarities existed between the roots of Bupleurum chinense DC(Bei Chaihu),B.falcatum(San-dao Chaihu) and B.scorzonerifolium Willd.(Nan Chaihu),but Nan Chaihu contains much lower total saponins than that in Bei Chaihu.The other species of Bupleurum demonstrated their different chemical distribution.The toxic species of B.longiradiatum can be easily differentiated from other spp.by comparison with the HPTLC images.Conclusion The survey showed that the main commodities of’Chaihu’in the domestic market can be attributed to’Bei Chaihu fingerprint-pattern’.The toxic species of B.longiradiatum can be easily differentiated from other spp.by comparison with the HPTLC images.
