Determination of geniposidic acid,chlorogenic acid and geniposide in Duzhong Pingya Tablet by RP-HPLC
- VernacularTitle:RP-HPLC测定杜仲平压片中京尼平苷酸、绿原酸、京尼平苷的含量
- Author:
Fengxian MA
;
Jinjin HE
;
Qin LI
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Duzhong Pingya Tablet;
geniposidic acid;
chlorogenic acid;
geniposide
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To estabish a RP-HPLC method for determing geniposidic acid、chlorogenic acid、geniposide in Duzhong Pingya Tablet(Folium Eucommiae).METHODS:Geniposidic acid,chlorogenic acid and geniposide were analysed by using C_(18) column with the mobile phase of methanol-water-aceacid(15∶85∶1.5) at the flow rate of 1 mL/min,the detective wavelength was set at 237 nm. RESULTS: The analytical had good linearity.The precision of geniposidic acid,chlorogenic acid and geniposide were 1.93%、1.50%、1.66%,respectively and the recoveries were 97.3%,97.2%,96.7%,respectively. CONCLUSION: This method is simple,quick and reproducible and could be used for the determination of three constituents in Duzhong Pingya Tablet.
