Amplification,purification and identification of human K562 cell cDNA library and its transformation into yeast cell
- VernacularTitle:人K562细胞cDNA文库的扩增、纯化、鉴定和酵母细胞转化
- Author:
Feng OUYANG
- Publication Type:Journal Article
- Keywords:
Leukemia;
K562 cell line;
cDNA library;
Diversity
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To Amplify,purify and identify the cDNA library of human K562 cell in E.coli DH5? and to transform it into yeast cell for subsequent target protein screening.Methods:The cDNA library of human K562 cell was amplified.Then the library plasmids were extracted and transformed into Y187 yeast cell.The results of transformation were verified by PCR and restriction enzyme analysis.Results:The cDNA library of human K562 cell was amplified successfully and the diversities were verified.The cDNA library of human K562 cell was also successfully transformed into Y187 yeast cell.Conclusion:Successfully amplification,purification and identification of the cDNA library of human K562 cell lay the foundation for the subsequent protein screening.
