Efficacy comparison of three primary cell culture methods for mature rat adipocytes
- VernacularTitle:大鼠成熟脂肪细胞原代培养方法比较
- Author:
Yan ZHANG
- Publication Type:Journal Article
- Keywords:
Adipocytes;
Cell culture;
Primary culture;
Rats
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To identify the best method for isolating and culturing mature rat adipocytes in vitro.Methods:Three different primary culture methods,i.e.organ culture,ceiling culture,and modified ceiling culture were used in the present study.Mature Sprague-Dawley rat adipocytes were cultured in vitro.The cells were observed under microscope and oil red O staining to compare the distinction of cells with different culture methods in the same condition.Rusults:Adipocytes were successfully cultured by the three primary cell culture methods.Cells cultured were highly homogenous.Modified ceiling culture method was the best in terms of the efficiency of culturing and cell morphology.Conclusion:Modified ceiling culture,is a long-term stable primary culture method for mature rat adipocyte,providing a good technique platform for the studies related to adipocytes.
