Establishment of rat model of epilepsy induced by lithium-repeated low-dose pilocarpine
- VernacularTitle:氯化锂-重复低剂量匹罗卡品致大鼠癫痫模型制作
- Author:
Xiaodong ZHANG
- Publication Type:Journal Article
- Keywords:
Lithium;
Repeated low-dose pilocarpine;
Epileptic model
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To estabhish rat model of epilepsy induced by the Lithium-repeated low-dose pilocarpine.Methods:A totol of 173SD male rats were divided into the normal control group,seizure-induced group and Chloral Hydrate group randomly.Lithium chloride was injected at a dose of 3 mEq/kg intraperitoneally(ip) 19h prior to the administration of pilocarpine.Rats were injected with pilocarpine,10mg/kg ip,every 30min,and this injection was repeated for 2~4 times.We then determined the rat activity,EEG,the percentage of rats exhibiting status epilepticus(SE),the mortality rate,the proportion of rats developing spontaneous seizures,the latency for the first spontaneous motor seizure,and the frequency of the spontaneous recurrent seizures.Results:The lithium-repeated low-dose pilocarpine resulted in SE after 1~4 injections;the average period between seizure and injection was 16min and the success rate was 73%.The mean dose of pilocarpine needed to induce SE was 25.9mg/kg.When SE was interrupted after 90min,mortality rate was below 10%,Spontaneous seizure occurred 2.4~5.7 times a day averagely,and average latency to the first spontaneous seizure was approximately 40days.All seizure incidence was over level Ⅲ and most were level Ⅳ/Ⅴ;EEG was similar to that of human temporal epilepsy;seizure of rats in control group was level 0.Conclusion:Lithium-repeated low-dose pilocarpine induced rats model of epilepsy is characterized by easy building,with high successful rate and lower mortality of the animal.This model has mimic features of human status epilepticus and temporal epilepsy in seizure behavior and electroencephalographic findings.
