Study on the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine against echinococcus multilocularis
- VernacularTitle:多房棘球绦虫重组BCG-Em14-3-3疫苗诱导小鼠脾细胞因子变化的研究
- Author:
Wengui LI
- Publication Type:Journal Article
- Keywords:
Echinococcus multilocularis;
Recombinant BCG-Em14-3-3 vaccine;
Cytokines
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the changes of splenocyte cytokines in mice induced by recombinant BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em) and challenge with Em protoscoleces.Methods:BALB/c mice were subcutaneously and intranasally vaccinated respectively,then challenged with Em protoscoleces at 8th week of vaccination and killed at 18th week of infection to get spleen.Splenocytes were separated to culture by stimulation with EmAg or ConA.The supernatants were collected to measure IL-2、IFN-?、TNF-?and IL-4 by ELISA Kits,and blank vector,BCG and PBS served as control.Results:In the groups of immunization,levels of IFN-?and TNF-? increased,but that of IL-4 decreased.The level of TNF-? in the intranasal group was higher than that in the subcutaneous group.Conclusion:TH1 response is induced in mice by rBCG-Em14-3-3 vaccine which is against the challenge of Em protoscoleces.
