Genotyping of 238 HBV strains by the methods of type-specific primers PCR
- VernacularTitle:型特异引物PCR法分析238株乙肝病毒基因型
- Author:
Aizhong ZENG
- Publication Type:Journal Article
- Keywords:
Hepatitis B virus;
Genotype;
Type-specific nucleotides;
Polymerase chain reaction(PCR)
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a set of suitable and reliable methods and to study the distribution of HBV genotypes.Methods:Type-specific primers were designed,and type-specific primers PCR was used to genotype the HBV strains.Results:A total of 218 out of the 238 strains were genotyped.Among them,49(68.3%) cases were genotype B(male 69.3%,female 65.3%),63(28.9%) cases were genotype C(male 28.3%,female 30.8%),6(2.8%) cases were mixtures of genotype B and C(male 2.4%,female 3.8%).No genotype A,D,E,F,G and H were found.Conclusion:Genotype B is the most dominant type for HBV strains and there is no relationship between the gender and HBV genotypes.
