Cloning of ICAT and its anti-cancer function
- VernacularTitle:ICAT的克隆及其抑癌作用
- Author:
Jing REN
- Publication Type:Journal Article
- Keywords:
ICAT;
Cloning;
Anti-tumor
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To Clone the cDNA of human ICAT,and express it in tumor cell,to investigate its anti-tumor effect.Method:the ICAT cDNA was amplified by RT-PCR,the ukaryotic expression plasmid pcDNA3.1-ICAT was constructed,MCF-7 and MDA-MB231 breast cancer cells were transfected by pcDNA3.1-ICAT using liposome and the cancer cell proliferation was estimated by MTT.Result:The product of RT-PCR was about 260bp,its sequence was the same as that reported,the transfection efficiency for MCF-7 and MDA-MB-23 cells was about 26%,and inhibiting efficiencies on the proliferation of the cancer cell transfected were 18.1% and 16.2% respectively.Conlusion:The cDNA of ICAT was cloned,and its expression in MCF-7 and MDA-MB-23 cells may inhibit the proliferation of these cancer cells.
