Experimental study on radiosensitivity enhancement effect of COX-2 inhibitor on breast cancer
- VernacularTitle:COX-2抑制剂对乳腺癌放射增敏作用的实验研究
- Author:
Fangxin LIU
- Publication Type:Journal Article
- Keywords:
Selective cyclooxygene-2 inhibitor;
Radiosensitivity;
Breast cancer;
Apoptosis
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the radiosensitivity enhancement effect of COX-2 inhibitor-Nimesulide on breast cancer cell line(MCF-7) in vitro and to initially disclosure its mechanism.Methods:The clonogenic assay was performed to determine the radiosensitizing effect of Nimesulide on MCF-7 cell line.The morphological changes of MCF-7 were observed by electron microscopy.The apoptosis and expressions of Bcl-2 and Bax were measured by flow cytometry(FCM).Results: Nimesulide at different concentration significantly enhanced the radiosensitivity of MCF-7 cells.in a dose-dependent manner,under the combined treatment of Nimesulide and radial the protein expression of Bax was up-regulated,while that of Bcl-2 was inhibited and the apoptpsis increased significantly.Conclusion:Cox-2 inhibitor could obviously enhance the radiosentivity of human breast cancer cell line MCF-7.
