Histocytological Observation of Somatic Embryogenesis in In-Vitro Cultured Dendrobium candidum wall. Ex Lindl.
- VernacularTitle:铁皮石斛离体培养体细胞胚发生的组织细胞学研究
- Author:
Chaomei PAN
;
Jiayun TONG
;
Danxia LIU
;
Aiqun HU
;
Shuqing YANG
- Publication Type:Journal Article
- Keywords:
DENDROBIUM CANDIDUM WALL.EX LINDL.;
MEDICINAL HERB ANIMAL RAISING;
TISSUE CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the somatic embryogenesis in in-vitro cultured Dendrobium candidum wall.Ex Lindl.(DCWL),and to supply evidence for its rapid propagation and germplasm preservation.Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants,N6 was used as the basic culture with phytohormone added,and fungal extracts as the elicitor.Protocorm-like bodies and callus of DCWL were used for induction culture.Results Somatic embryogenesis in in-vitro cultured DCWL derived from the epithelial cells or inner cells of callus of DCWL.Conclusion A large amount of buds can be obtained by the induction and culture of protocorm-like bodies and callus of DCWL.
