Determination of Lasiokaurin in the Stems and Leaves of Rabdosia nervosa by HPLC
- VernacularTitle:HPLC法测定显脉香茶菜中毛栲利素的含量
- Author:
Youheng GAO
;
Zhixiong WEI
;
Honghua CUI
;
Shenglin LIANG
;
Hongfei CAI
- Publication Type:Journal Article
- Keywords:
RABDOSIA NERVOSA/chemislry;
LASIOKAURIN/analysis;
CHROMATOGRAPHY,HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC method for the determination of Lasiokaurin in the stems and leaves of Rabdosia nervosa.Methods HPLC was performed on a Kromsil C18 reversed-phase column(250mm?4.2mm,5?m).All of the reference substances and samples were separated with the mobile phase of methanol :water(55:45) under isocratic elution for 30min,flow rate was 1.0ml/min,the detection wavelength was 234nm,and the column temperature was 30℃.Results The content of Lasiokaurin was 0.005mg/g in the stems and 0.372mg/g in the leaves,the content in the stems were almost 1/75 times as much as that in the leaves.The average recovery of Lasiokaurin was 96.21%.Conclusion This developed method,which is simple and with good precision,high sensitivity and selectivity,can be used for quality evaluation of Lasiokaurin in Rabdosia nervosa.
