Simultaneous Determination of 4 Anthraquinones in Shugan Quzhi Capsules by RP-HPLC
- VernacularTitle:RP-HPLC法同时测定舒肝祛脂胶囊中4种大黄蒽醌的含量
- Author:
Lan CUI
;
Furong AN
;
Linghong XIA
- Publication Type:Journal Article
- Keywords:
rhein;
emodin;
chrysophanol;
physcion;
Shugan Quzhi capsule;
RP-HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP-HPLC method for simultaneous quantitative determination of 4 anthraquinones in Shugan Quzhi capsules. Methods A RP-HPLC method was used with a Diamonsil C18 column (5 ?m,4.6 mm?150 mm). The mobile phase was composed of methanol and 0.1% H3PO4 water solution (77∶23,v/v). The wavelength of UV detector was 254 nm and column temperature was 30 ℃. Flow rate was 1.0 mL/min and 20 ?L was injected every time. Results The linear ranges of rhein,emodin,chrysophanol and physcion were 0.083 2～2.08,0.100 8～2.52,0.291 2～7.28 and 0.088～2.20 ?g/mL,respectively. Their average recoveries were 97.3%,96.9%,96.5% and 95.9%,respectively. Conclusion This method was sensitive,repeatable and suitable to determine the contents of 4 anthraquinones in Shugan Quzhi capsules.
