Determination of Alkaloids in Zanthoxylum Nitidum by Ultraviolet Spectrophotometry
- VernacularTitle:紫外分光光度法测定两面针中总生物碱的含量
- Author:
Wei ZHANG
;
Shao LIU
;
Guixia HE
- Publication Type:Journal Article
- Keywords:
Zanthoxylum nitidum;
Alkaloids;
content determination;
ultraviolet spectr-ophometry
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a simple method for determination the Alkaloids contents from Zanthoxylum nitidum. Methods Adopting nitidunechloride as reference,alkaloids in Zanthoxylum nitidum were by ultraviolet spectrophotometry at 329 nm. Results The liner arrange was 3～8 ?g/mL,regression equation:Y=97.75X -0.025 5,r =0.999 1 (n=6). The mean recovery of Nitidunechloride was 99.46%,RSD=0.93% (n =5). Conclusion The method performed is accurate and simple. The reproducibility and rate of extraction are also desirable.
