Effect of Elemene on Transcription Factor ELK1 and Its Target Gene of Human Cervix Cancer Hela Cell
- VernacularTitle:榄香烯乳对人宫颈癌Hela细胞转录因子ELK1及其靶基因的影响
- Author:
Xiaojun CHEN
;
Ligang GU
;
Peiwen LI
- Publication Type:Journal Article
- Keywords:
elemene;
Hela cell;
ELK1
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of elemene on transcription factor ELK1 and its target gene in human cervix cancer Hela cell. Methods The cell proliferation was assessed by MTT assay. The luciferase activity of transcription factor ELK1 was determined by the Dual-Luciferase Reporter Assay System. The protein expression of phosphorated ELK1 and its target gene c-fos were determined by Western Blot. Results Elemene can remarkably inhibited the growth of Hela cell and its IC50 was 80.6 ?g/mL. The luciferase activity of transcription factor ELK1 in Hela cells treated with elemene was inhibited. The protein expression of phosphorated ELK1 and its target gene c-fos in Hela cells treated with elemene were down-regulated. Conclusion Elemene can inhibit human cervix cancer Hela cell proliferation,which may be related with suppression of c-fos gene through inhibiting expression of phosphorated ELK1.
