Determiation of notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Xinning Tablet by HPLC-ELSD
- VernacularTitle:HPLC-ELSD测定心宁片中三七皂苷R_1、人参皂苷Rg_1、人参皂苷Rb_1的含量
- Author:
Xiangdong FENG
;
Haixin HUANG
;
Guangwei GAO
;
Jinxia ZHANG
;
Xia SU
- Publication Type:Journal Article
- Keywords:
Xinning Tablet;
notoginsenoside R_1;
ginsenoside Rg_1 and Rb_1;
HPLC-ELSD
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish the method of determining notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Xinning Tablet(Radix et Rhizoma Salvae Miltiorrhizae,Radix et Rhizoma notoginseng,Flos Carthami,Rhizoma Chuanxiong,ect).METHODS:HPLC-ELSD was used to determine notoginsenoside R_1,ginsenoside Rg_1 and Rb_1 in Xinning Tablet.The separatrion was performed on C_ 18 colunm with acetonitrile and water being used as a gradient program at 35 ℃.The elution program was(0-5 min,20%-25% acetonitrile;5-20 min,25%-45% acetonitrile),drift tube temperature was at 70 ℃,gas flow rate of 2.0 L/min.RESULTS:3 saponins were separated well.Average recoveries were 102.32% for notoginsenoside R_1 100.73% for ginsenoside Rg_1;101.40% for ginsenoside Rb_1,respectively.CONCLUSION:The method is simple and rapid and with satisfactory results and is suitable for quality control of Xinning Tablet.
