Compound analysis of Alisma orientalis (Sam.) Juzep.by HPLC-EMI-MS
- VernacularTitle:高效液相色谱-电喷雾-质谱法分析泽泻中的活性成分
- Author:
Xinfeng ZHAO
;
Yuqing SUN
- Publication Type:Journal Article
- Keywords:
HPLC-EMI-MS;
Alisma orientalis(Sam.)Juzep.;
alisol B;
alisol B-23-aceate;
alisol C-23-aceate;
alisol C;
16-oxoalisol A;
11-deoxyalisol C
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a high performance liquid chromatography-electrospray ionization-mass spectrometry(HPLC-EMI-MS)analysis approach of Alisma orientalis(Sam.)Juzep.METHODS:A Zorbax SB-C_ 18 column was used under the gradient elution condition with acetonitrile-H_2O.The MS detection was performed on an Agilent ion trap mass spectrometer with an elctrospray ionization source under the positive ion detection mode.RESULTS:Alisol B,alisol B-23-aceate,alisol C-23-aceate,alisol C,16-oxoalisol A and 11-deoxyalisol C were identified by their MS and MS2 fragments.The MS2 fragments of the 6 compounds were completed.CONCLUSION:The total ion current chromatogram of Alisma orientalis(Sam.)Juzep.was more characteristic than of the UV chromatogram.6 bioactive compounds can be simultaneously analyzed by the HPLC-EMI-MS method established in this experiment and it is a good quality control of Alisma orientalis(Sam.)Juzep.
