Establishment of proteomic techniques and two-dimensional electrophoregram of human hepatocellular carcinoma cell line QGY-7701
- VernacularTitle:人肝癌细胞株QGY-7701蛋白质组学研究技术及二维电泳图谱的建立
- Author:
Yonghua YUAN
- Publication Type:Journal Article
- Keywords:
Human hepatocellular carcinoma cell line QGY-7701;
Proteomics;
2-dimensional gel electrophoresis(2-DE)
- From:
Journal of Chongqing Medical University
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish proteomic technique system of human hepatocellular carcinoma cell line QCY-7701. Method:Two different lysis buffer were taken to extract cell proteins. After two-dimensional gel electrophuresis (2-DE) and PDQuest analysis, 10 good-matched protein spots were chosen to be identified by MALDI-TOF-MS. Results:A steady 2-DE electrophregram of hepatocellular carcinoma cell line QGY-7701 was established. Compared with lysis bufferⅠ,protein quantities extracted from lysis bufferⅡincreased by 25%;protein spots in 2-DE gel increased by 32%. 9 out of 10 candidate protein spots were successfully identified (P
