Comparison Study on the Associated Gene Expression in Thymus-dependent Lymphocyte of COPD Patients with Pulmonary Qi Deficiency Syndrome and Pulmonary Yin Deficiency Syndrome
- VernacularTitle:慢性阻塞性肺疾病肺气虚证和肺阴虚证患者T淋巴细胞相关基因表达对比研究
- Author:
Zegeng LI
;
Jiabing TONG
;
Bo PENG
- Publication Type:Journal Article
- Keywords:
chronic obstructive pulmonary disease;
pulmonary qi deficiency syndrome;
pulmonary yin deficiency syndrome;
thymus-dependent lymphocyte;
gene expression;
gene chip
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the associated gene expression in thymus-dependent lymopholyte of the COPD patients with pulmonary qi deficiency syndrome(PQDS)and pulmonary yin deficiency syndrome (PYDS)by gene chips.Methods Collect peripheral blood of patients with PQDS and PYDS(as experimental samples)and of nomal man(as control samples).By Ficoll method,peripheral blood lymphocyte was collected,then thymus-dependent lymphocyte were extracted and purified by flow cytometry.Total RNA were extracted by one-step technique and purified.Then,synthesis double strand cDNA template from total RNA,transcription of cRNA probe with biotin labeling,subsequently,cRNA sample were fragmented. The gene chip(Human Genome U133 Plus 2.0 Array,38500 genes)was hybridized and scanned.Then fluorescent signal value of gene expressing was obtained,and differential expression genes were sifted. Results There were 45 genes expressed differently among PQDS and normal man,including up-regulated 41 and down-regulated 4;32 genes expressed differently between PYDS and normal man,including up-regulated 19 and down-regulated 13;5 up-regulated genes expressed differently between PQDS and PYDS with normal man.Conclusion Gene chip can be applied to study gene expression profiles effectively and to screen PQDS and PYDS associated genes.
