Effect of Yishentongfei Decoction on MMP-9 and TIMP-1 in Lung Tissue of Rat Model of Chronic Obstructive Pulmonary Disease
- VernacularTitle:益肾通肺汤对实验性慢性阻塞性肺疾病大鼠肺组织MMP-9和TIMP-1表达的影响
- Author:
Jie SUN
;
Liwen WU
;
Zhaoning ZHANG
- Publication Type:Journal Article
- Keywords:
Yishentongfei Decotion;
COPD;
MMP-9;
TIMP-1;
LPS
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the role of prevention and treatment of Yishentongfei decoction in COPD by measuring the expression of MMP-9 and TIMP-1 in lung tissue.Methods 36 male Wistar rats of rat model with chronic obstructive pulmonary disease was established by exposure to cigarette smoke daily for 30 days and injection of LPS in trachea twice.The rats were randomly separated into three groups,the normal group,the model group and the treatment group.Total cell counts and neutrophil counts in BALF were examined.The expression of MMP-9 and TIMP-1 in lung tissue was measured by means of immunohistochemistry(SP).Lung tissue section stained by HE was observed to study the morphological alternations.Result The expression of MMP-9 and TIMP-1 in lung tissue of the model rats significantly increased compared with the normal rats(P
