Content Determination of Piperine in Biba Zhitong Tincture by HPLC
- VernacularTitle:高效液相色谱法测定荜茇止痛酊中胡椒碱的含量
- Author:
Zheng YAO
;
Jiangang GU
;
Jinong CHU
- Publication Type:Journal Article
- Keywords:
Biba Zhitong Tincture;
Piperine;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of content determination of Piperine in Biba Zhitong Tincture.Methods HPLC was adopted in determining the content of piperine in Biba Zhitong Tincture.C18 chromatographic column was used with the column temperature being the room temperature.The mobile phase was tetrahydrofuran-methanol-water(V∶V∶V=30∶30∶50)with the velocity of 1.0 mL/min.The detection wavelength was 343nm,and the sampling volume was 10?L.External standard method was chosen for content calculation.Results The inner peak area of piperine(8.3～41.5 mg/L)showed fine linear relation with mass concentration of control group.Its regression equation was A=3413186?-7467.7,r= 0.999 8,with the average recovery ration being 99.7%,and RSD=1.8%(n=6).Conclusion The method is simple,convenient,good reproducibility and non-interference,and can effectively control the quality of the preparation.
