Determination of Rutin in Herba Cirsii by HPLC
- VernacularTitle:高效液相色谱法测定小蓟中芦丁含量
- Author:
Yu CHEN
- Publication Type:Journal Article
- Keywords:
Cirsium setosum(Willd.)MB.;
rutin;
content determination;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective HPLC was used to establish a method for determination of rutin in herba crisii. Methods Lichrospher C18 column(4.6 mm?200 mm,5?m)was used with methoanol-acetic acid(35∶65) as mobile phase,flow rate being 1.0 mL/min,column temperature at 30℃,detection wavelength at 254 nm.Results The content of rutin in herba crisii was 0.5808 mg/g.Conclusions The method is proved to be simple,rapid,and accurate.It can be applied to content determination of rutin in herba crisii.
