Determination of Puerarin in Jiangzhijianfei Granules by HPLC
- VernacularTitle:高效液相色谱法测定降脂减肥颗粒中葛根素含量
- Author:
Zhongkui GUO
;
Fenghui HUANG
;
Xin LI
- Publication Type:Journal Article
- Keywords:
HPLC;
Puerarin;
Jiangzhijianfei Granules
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of Puerarin in Jiangzhijianfei Granules by high performance liquid chromatography.Methods The determination was conducted by HPLC using a Shim-pack-C18 column(5?m,150 mm?4.6 mm)and a mobile phase of methanol-0.1%Phosphoric acid water(25.3∶74.7).The flow rate was 0.9 mL/min and the detection wavelength was 250 nm.Results Good linear relationship between area and amount was noted for 0.114～0.570?g of Puerarin,with a correlation coefficient of 0.999 6.The average recovery of was 98.74%,RSD was 0.67%.Conclusion The established method is simple,accurate,sensitive and can be applied to the quality control of the preparation.
