Effect of Cryptoporus polysaccharide on eotaxin mRNA and TNF-? mRNA expression in lung tissue of sensitized mice
- VernacularTitle:隐孔菌多糖对致敏小鼠肺组织趋化因子mRNA和-肿瘤坏死因子mRNA表达的影响
- Author:
Li CHEN
;
Yicheng XIE
;
Chuankui KE
;
Qiangmin XIE
- Publication Type:Journal Article
- Keywords:
Cryptoporus polysaccharide (CP);
eotaxin mRNA;
TNF-? mRNA;
asthma
- From:
Chinese Traditional and Herbal Drugs
1994;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the time-course relationship of eotaxin mRNA and TNF-? mRNA expression in the lung tissue of sensitized mice after antigen challenge and the effects of Cryptoporus polysaccharide (CP) on these expression. Methods Eotaxin mRNA and TNF-? mRNA expression was determined by semi-quantitative RT-PCR. The function implications of eotaxin mRNA and TNF-? mRNA expression were examed by detecting the number of total leucocyte and eosinophils in bronchoalveolar lavage fluid (BALF). Results Peak level of TNF-? mRNA expression appeared at 8 h and eotaxin mRNA expression at 24 h in the lung tissue of sensitized mice after antigen challenge. Compared with the control group, total numbers of leukocyte and eosinophil in BALF increased in sensitized mice. CP 3, 10, and 30 mg/kg inhibited eotaxin mRNA and TNF-? mRNA expression, and inflammatory cells accumulation in airway of the sensitized mice in a dose-dependent manner. Conclusion The mechanism of inhibitory effects of CP on eotaxin mRNA and TNF-? mRNA expression may be related to the anti-allergic inflammation.
