IMMUNOHISTOCHEMICAL STUDIES OF EGF AND IL-2 POSITIVE CELLS IN RAT SUBMANDIBULAR GLAND DURING HEROIN DEPENDENCE PERIOD
- VernacularTitle:海洛因依赖期间大鼠下颌下腺EGF及IL-2阳性细胞的免疫组织化学研究
- Author:
Yun HU
;
Wenmei LIANG
- Publication Type:Journal Article
- Keywords:
EGF;
IL-2;
Heroin dependence;
Submandibular gland;
Immunohistochemistry;
Rat
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of heroin on EGF and IL-2 positive cells in the submandibular gland of rats. Methods Normal SD rats were divided into normal control group (NGC),saline control group (SCG)and heroin dependence group (HDG). The heroin dependent model of rats was established by subcutaneous injection of heroin, and submandibular glands were excised on the following days respectively: the 10th, 17th, 24th, 31th and 38th. Immunohistochemical SABC method, image analysis and cells calculation were used in the study. Results 1. As compared with those of the NCG, the number of EGF and IL-2 positive cells and the intensity of staining in SCG didn't change obviously. 2. During heroin dependence, the immunohistochemical staining showed that the number of EGF and IL-2 positive cells increased obviously(P
