REVERSAL OF RESISTANCE TO ADRIAMYCIN IN MCF-7/ADM CELLS BY REALGAR IN COMBINATION WITH ?-ELEMENE
- VernacularTitle:雄黄与?-榄香烯联合用药逆转MCF-7/ADM细胞对阿霉素耐药性的研究
- Author:
Jun HU
;
Jinyao ZHAO
;
Wei JIN
;
Jin YANG
;
Peima YANG
- Publication Type:Journal Article
- Keywords:
Realgar;
?-elemene;
MCF-7/ADM cell line;
Resistance to ADM;
MTT assay;
Flow cytometry
- From:
Acta Anatomica Sinica
2002;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the reversal effect of adriamycin (ADM) resistance in human breast cancer cell line MCF-7/ADM by realgar (REA) combined with ?-elemene (?-ELE), two types of anticancer drugs. Methods The sensitivity to ADM of MCF-7/ADM cells was studied by MTT assay.The intracellular accumulation of ADM in MCF-7/ADM cells was observed by fluorescent-spectrophotometry. Expressions of P-GP protein and Bcl-2 protein were detected by flow cytometry. Results The combined treatment of REA and ?-ELE could significantly enhance the cytotoxic effect of ADM on MCF-7/ADM cells to 4.2 fold as compared with the treatment of REA or ?-ELE respectively (P
