Development of Chromatographic Fingerprint of Rhizoma Alismatis Orientalitis by HPLC-ELSD
- VernacularTitle:泽泻饮片的HPLC-ELSD指纹图谱研究
- Author:
Liping GONG
;
Shaoyun WANG
;
Ke ZHANG
- Publication Type:Journal Article
- Keywords:
Rhizoma Alismatis Orientalitis;
Fingerprint;
HPLC;
Evaporative light scattering detector (ELSD)
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective A fingerprint of Rhizoma Alismatis Orientalitis' decoction pieces was established by HPLC-ELSD. Methods The gradient elution mode was applied in chromatographic separation by mobile phase of methanol and water at flow rate of 0.5 mL? min-1 in a Diamonsil TM C18 chromatographic column (250 mm ? 4.6 mm, 5 ? m), and recording time was 70 minute. The parameters of evaporative light scattering detector were set as follows: the temperature of the drift tube was 82.5 ℃ and gas flow rate was 1.9 L ? min-1.The data was analyzed with cluster analysis and similarity calculation software. Results The fingerprint showing 15 common peaks was obtained. The results of cluster analysis and similarity calculation showed there existed differences in fingerprints of 16 batchers of medicinal slices of Rhizoma Alismatis Orientalitis. Conclusion The method is effective and reproducible for the quality control of medicinal slices of Rhizoma Alismatis Orientalitis.
