Determination of Ginsenoside-Rd Concentration in Human Urine by Liquid Chromatography-Mass Spectrum
- VernacularTitle:液相色谱-质谱联用测定人尿中人参皂苷-Rd的浓度
- Author:
Li SUN
;
Yuanhui DENG
- Publication Type:Journal Article
- Keywords:
Ginsenoside-Rd;
Liquid chromatography-mass spectrum;
Urine concentration
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of liquid chromatography-mass spectrum for the determination of /Ginsenoside-Rd in human urine. Methods With the gentiopicroside as internal standard, the dilution method was adopted to treat the urine samples. Electrospmy ionization(ESI)source was applied and operated in the positive ion mode. Multiple reaction monitoring(MRM)mode was used to detect Ginsenoside-Rd content. Results By this method the linearity limit of Ginsenoside-Rd is 30.3～ 10 100 ng? mL-1, the lower quantitative limit is (30.3? 2.16) ng? mL-1, and the inter-and intra-day precision (RSD) was less than 10 % . Conclusions This is an accurate, sensitive, specific and convenient method, which can be used for the determination of Ginsenoside-Rdin urine of the healthy human.
