Determination of Vitexin in Cajanus cajan(L.) Millsp. by HPLC
- VernacularTitle:岭南常用中药木豆叶中牡荆苷的HPLC含量测定
- Author:
Gongying LI
;
Hongmei TANG
;
Zhenwen QIU
;
Yuechun HUANG
;
Yingna ZHANG
- Publication Type:Journal Article
- Keywords:
Cajanus cajan (L.) Millsp.;
Vitexin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a quantitative method to determine vitexin in Cajanus cajan (L.) Millsp. Methods The chromatographic conditions were as follows: column C18(250 mm? 4.6 mm, 5 ? m) , mobile phase being MeOH ∶ 1 % HAC (25 ∶ 75) for 20 mins and then being MeOH ∶ 1 % HAC (30 ∶ 70) after 20 mins, flow rate at 1.0 mL/min, and wavelength at 339 nm. Results The good linearity of this method was in the range of 0.104 4～ 0.522 ? g (r=0.999 4), and the average recovery of vitexin was 100.11 % ( RSD =0.63 % ).Conclusion The method is simple and sensitive with good stability, it can be suitable for the quality control of vitexin in Cajanus cajan (L.) Millsp..
