Determination of Stilbene Glycoside in Yangyan Pills by HPLC
- VernacularTitle:高效液相色谱法测定养颜丸中二苯乙烯苷的含量
- Author:
Xinyu CHEN
;
Yifen LUO
- Publication Type:Journal Article
- Keywords:
Yangyan Pills;
2, 3, 5, 4'-teterahydroxystibene-2-O-?-D-glucoside;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the determination of 2, 3, 5, 4'-teterahydroxystibene-2-O-?-D-glucoside in Yangyan Pills. Methods HPLC was carried out on a C18 column, using mobile phase of acetonitril-water(25 ∶ 75), and detection wavelength was 320nm. Results The 2, 3, 5, 4'-teterahydroxystibene-2-O-?-D-glucoside sample showed a good linear relationship at the range of 0.05～ 0.40 mg/mL, r=0.999 9. The average recovery was 99.7 % (n=9), and RSD was 0.31 % (n=9). Conclusion The method was simple, available and accurate.
