Effects of Bingcha Embolus on the proliferation,apoptosis and cell cycle of astrocytes
- VernacularTitle:冰茶栓对星形胶质细胞增殖、凋亡及细胞周期的影响
- Author:
Jigui YAN
;
Yeling TONG
;
Guonong HE
;
Lixia YU
;
Zeshi WANG
- Publication Type:Journal Article
- Keywords:
Bingcha Embolus;
serum pharmacology;
astrocytes;
flow cytometry;
rat
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects of Bingcha Embolus on the proliferation, apoptosis and cell cycle of astrocytes in vitro in terms of serum pharmacology. METHODS: The cerebral cortex astrocytes of neonatal Sprague-Dawley rats were cultivated in vitro, and the purified astrocytes were randomly devided into Bingcha Embolus groups (high dose, middle dose, low dose) and control group after being identified by means of immunocytochemical method. After the incubation of 48 h with sera, MTT was employed as a proper method to detect the proliferation of astrocytes. The ratio of apoptosis and different cell cycle phases were measured by Flow Cytometry. RESULTS: The results of MTT showed that the cell number of Bingcha Embolus groups (high dose, middle dose) were obviously fewer than that of the control group (P
