Determination of quercetin, kaempferol,isorhamnetin and flavonoids in Compound Ginkgo Granules by HPLC
- VernacularTitle:HPLC测定复方银杏颗粒中槲皮素、山柰素、异鼠李素及总黄酮醇苷含量
- Author:
Fei XU
;
Tulin LU
;
Hui XIE
;
Chunqin MAO
- Publication Type:Journal Article
- Keywords:
Compound Ginkgo Granules;
quercetin;
kaempferol;
isorhamnetin;
flavonoids;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a method for determining total flavonoids in Compound Ginkgo Granules so as to control the quality. METHODS: HPLC conditions: YWG-C_ 18 column, mobile phase: V(MeOH)∶V(1%HAC)=50∶50, flow rate: 1.0 mL/min, detection:?=360 nm, column temperature: 25 ℃. RESULTS: The calibration curve of quercetin、 kaempferol and isorhamnetin was linear in range of 0.057 2 -0.286 0 ?g, 0.074 0 -0.370 0 ?g, 0.029 4 -0.147 0 ?g, respectively. The average recovery was 98.39% , and RSD was 1.25 (n=6). CONCLUSION: This method is rapid, accurate and reproducible and could be used for quality control of Compound Ginkgo Granules.
