Effect of Ligustrazine on Cell Apoptosis and Expression of bcl-2 and p53 in Rats Hippocampal Neurons After Anoxia
- VernacularTitle:川芎嗪对缺氧大鼠海马神经元凋亡及bcl-2、p53基因表达的影响
- Author:
Yonghua ZHAO
;
Xiaoxing LUO
;
Yude LIU
;
Yuping HE
- Publication Type:Journal Article
- Keywords:
LIGUSTRAZINE/pharmacology;
CEREBRAL ISCHEMIA/TCD therapy;
GENE EXPRESSION REGULATION;
APOPTOSIS;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2004;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】To observe the effect of ligustrazine(Lig) on cell apoptosis and expression of bcl-2 and p53 in rats hippocampal neurons after anoxia,and to explore its protective mechanism.【Methods】Primary culture technique of rat hippocampal neurons was used to establish the model of anoxia.At the 14th day of the culturing,the cultured cells were divided into model group,and high-,middle-and low-dosage ligustrazine (800,200 and 50 ?g/mL respectively) groups,which were exposed to anoxia environment for continuous 1.5 hours.Flow cytometer was used for the quantitative analysis of apoptosis rate of neurons,and in-situ hybridization was used to detect the bcl-2 and p53 mRNA expression of hippocampal neurons.【Results】In middle-dosage ligustrazine group after exposure to anoxia environment for 1.5 hours,the apoptosis rate and the bcl-2 and p53 mRNA expression of hippocampal neurons differed from those in other groups(P
