Identification and cultivation of neural stem cells in neonatal rats in vitro
- VernacularTitle:新生大鼠神经干细胞的体外培养和鉴定
- Author:
Qin YANG
- Publication Type:Journal Article
- Keywords:
Neural stem cells;
Cell culture;
Neonatal rat
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the identification and cultivation of neural stem cells in neonatal rats in vitro.Methods:Cell culture in serum-free medium,indirect immunofluorescence cytochemistry were used.Results:The neural stem cells isolated in newborn rats had the potential of forming clones and serial passage,expressing neuroepithelial stem cell protein(nestin)and differentiating into neurons and astrocytes and oligodendrocytes.Conclusion:Neural stem cells maintain their undifferentiated features and have the capacity of self-renewal,proliferation and pluripotentiality.
