Effect of varicocele on expression of HIF-1? and VEGF in germ cells of adolescent rats
- VernacularTitle:精索静脉曲张对青春期大鼠睾丸生精细胞HIF-1?和VEGF表达的影响
- Author:
Tianbing LV
- Publication Type:Journal Article
- Keywords:
Varicocele;
Germ cell;
Hypoxia-inducible factor-1?;
Vascular endothelial growth factor
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effect of varicocele(VC)on Expression of hypoxia-inducible factor-1?(HIF-1?)and vascular endothelial growth factor(VEGF)in germ cells of adolescent rats,and investigate the mechanism of the infertility resulting from varicocele.Methods:The varicocele model was created by partial ligation of the left renal vein in adolescent male Wistar rats,a total of 50 rats were randomly divided into four groups,namely varicocele group,sham operation group(SOG),At 30 days and 60 days were completed,left orchiectomy was performed the expression of HIF-1? and VEGF were detected by immunohistochemical method.Results:The expression of HIF-1? and VEGF in left testes of varicocele group is significantly higher than of sham operation group.With the duration of varicocele mode1,HIF-1? and VEGF expression in experiment group was decreased,but was higher compared with that in control group.Conclusion:Experimental varicocele could change Expression of hypoxia-inducible factor-1?(HIF-1?)and VEGF in germ cells of adolescent rats.
