Injury of alveolar type Ⅱ cell and JNK signal transduction induced by hydrogen peroxide
- VernacularTitle:氧化应激对肺泡Ⅱ型上皮细胞的损伤作用及JNK信号转导机制
- Author:
Yueqiang FU
- Publication Type:Journal Article
- Keywords:
Alveolar epithelial type Ⅱ cell;
Apoptosis;
c-jun NH2-terminal kinase;
Reactive oxygen species
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate survival and apoptotic responses of alveolar epithelial typeⅡcells(ATⅡ cell)under oxidative stress and to discuss the regulation mechanism mediated by c-jun NH2-terminal kinase(JNK).Methods:Primary rat alveolar epithelial typeⅡcells were attacked by Hydrogen peroxide(H2O2)and cells were pretreated with SP600125 in some groups.Cell viability,apoptotic rate and the expression of phosphorylated JNK(p-JNK)were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay,flow cytometry and western blot analysis,respectively.Results:Compared with control group,decreased cell viability and increased apoptotic rate in ATⅡ cells occurred in time-dependent manner when treated with 500?mol/L H2O2 in different time.H2O2 induces phosphorylation of JNK.SP600125 enhanced cell viability and decreased apoptotic rate after H2O2-exposure.Conclusion:Apoptosis could be induced by H2O2 in ATⅡ cells in time-dependent manner.JNK signaling pathway may play a proapoptotic role in the regulation of apoptosis induced by H2O2.
