The primary study about the relationship of FBN2 gene and lung cancer cell lines
- VernacularTitle:FBN2基因与肺癌细胞关系的初步探讨
- Author:
Hong CHEN
- Publication Type:Journal Article
- Keywords:
FBN2;
Lung cancer cell lines;
Methylation;
5-Aza-cdR
- From:
Journal of Chongqing Medical University
2007;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the gene expression and aberrant methylation of FBN2 on lung cancer cell lines.Methods:Three lung cancer cell lines HCC366,H1299 and H2195 were cultured.mRNA was analysed by RT-PCR and methylation status was also investigated by methylation specific PCR.The loss of FBN2 expression cell lines were treated with the demethylating agent,5-aza-2'-deoxycytid-ine(5-aza-cdR).Results:The expression of FBN2 was detected in NHBEC and H2195,whereas it was not detected in HCC366 and H1299 which showed aberrant methylation of FBN2.FBN2 expression was restored after treatment with 5-aza-cdR.Conclusion:Methylation and silencing of FBN2 in tumor cells may play an important role in carcinogenesis of lung cancer.
